This Pyruvate Decarboxylase Assay Kit is a quantitative assay kit for measuring Pyruvate Decarboxylase activity in animal and plant tissues, cells, bacteria, serum, and plasma.
Applications
Functional Studies
Assay Type
Quantitative
Principle of Assay
Pyruvate decarboxylase converts pyruvate to acetaldehyde, which is subsequently reduced by alcohol dehydrogenase with NADH consumption. The decrease in absorbance at 340 nm is used to calculate activity.
Platform
96-Well Microplate, Microcuvette
Sample Type
Animal tissues, plant tissues, cells, bacteria, serum, and plasma
Assay Time
30-60 minutes
Components
Extraction Buffer, Reagent I, Reagent II, Reagent III, Reagent IV, Reagent V
Storage
Shipped at 4°C. Store at 4°C in the dark. Do not use past expiration date!
General Notes
A 96-well UV microplate or microquartz cuvette is required for this assay.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Publishing research using Pyruvate Decarboxylase Assay Kit (A319725)? Please let us know so that we can list the citation on this page.
