The antibody detects endogenous level of Rb only when phosphorylated at serine 795.
Applications
WB, IHC, ICC/IF
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of serine 795 (P-S-S(p)-P-L) derived from Human Rb.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
p105-Rb, p110-RB1, pp110, pRb, RB1, Retinoblastoma-associated protein
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Rb (Phospho-Ser795) Antibody #11130 (left) or the same antibody preincubated with blocking peptide (right).
![Immunohistochemistry - Anti-Rb1 Antibody [1F8] (A249832) - Antibodies.com](https://cdn.antibodies.com/image/catalog/249/A249833_1.jpg?profile=product_alternative)
