The antibody detects endogenous level of RB only when phosphorylated at serine 811.
Applications
WB, ICC/IF
Dilutions
Western blotting: 1:500 - 1:2000 Immunofluorescence: 1:50 - 1:200
Reactivity
Human, Mouse, Rat
Immunogen
A phospho specific peptide corresponding to residues surrounding S811 of human RB.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
p105-Rb, p110-RB1, pp110, pRb, RB1, Retinoblastoma-associated protein