Reactive Oxygen Species (ROS) Detection Fluorometric Assay Kit
Description
This Reactive Oxygen Species Detection Fluorometric Assay Kit is a quantitative assay kit for measuring Reactive Oxygen Species in cells.
Applications
Functional Studies
Assay Type
Semi-Quantitative
Principle of Assay
DCFH-DA enters cells and is hydrolyzed by intracellular esterases to nonfluorescent DCFH, which is oxidized by reactive oxygen species to fluorescent DCF. The fluorescence intensity reflects intracellular ROS levels.
Platform
Flow Cytometer, Fluorescence Microscope
Sample Type
Cultured cells
Assay Time
30-60 minutes
Components
DCFH-DA (10 mM)
Storage
Shipped at 4°C. Store at -20°C in the dark. Refer to the Protocol Manual for storage conditions of individual components. Do not use past expiration date!
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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