Recombinant Human CD3 delta Protein (6xHis Tag) + CD3 epsilon Protein (Fc Tag)
Description
Consists of CD3D (Phe22-Ala105) with a C-terminal 6xHis tag and CD3E (Asp23-Asp126) with a C-terminal human Fc tag. The human Fc tag consists of amino acids Glu99-Ala330.
Applications
SDS-PAGE
Expression System
HEK293 cells
Nature
Recombinant
Protein Species
Human
Protein Length
Protein fragment.
Sequence
CD3D (Phe22-Ala105) + 6xHis Tag and CD3E (Asp23-Asp126) + hFc (Glu99-Ala330)
Tag
C-terminal 6xHis Tag and C-terminal Human Fc Tag
Molecular Weight
The protein has a predicted molecular mass of 10.4 and 37.9 kDa after removal of the signal peptide. The apparent molecular mass of CD3D-His and CD3E-hFc is approximately 35-55 kDa due to glycosylation.
Conjugate
Unconjugated
Purity
> 95% by SDS-PAGE and Coomassie blue staining.
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
SDS-PAGE - Recombinant Human CD3 delta Protein (6xHis Tag) + CD3 epsilon Protein (Fc Tag) (A318035)
Recombinant Human CD3 delta Protein (6xHis Tag) + CD3 epsilon Protein (Fc Tag) (A318035) on SDS-PAGE under reducing conditions.
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