Recombinant Human CD30-L Protein (Fc Tag & 6xHis Tag)
Applications
ELISA, SDS-PAGE
Expression System
HEK293 cells
Nature
Recombinant
Protein Species
Human
Protein Length
Protein fragment.
Sequence
mFc (Pro99-Lys330) + CD30 Ligand (Gln63-Asp234) + 6xHis Tag
Tag
N-terminal Mouse Fc Tag and C-terminal 6xHis Tag
Molecular Weight
The protein has a predicted molecular mass of 48.0 kDa after removal of the signal peptide. The apparent molecular mass of mFc-CD30 Ligand-His is approximately 53-70 kDa due to glycosylation.
Conjugate
Unconjugated
Purity
> 95% by SDS-PAGE and Coomassie blue staining.
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Recombinant Human CD30-L Protein (Fc Tag & 6xHis Tag) (A318369)
ELISA plates were pre-coated with 2 µg/ml (100 µl/well) Recombinant Human CD30 Protein (6×His Tag) (A318247) which can bind Recombinant Human CD30-L Protein (Fc Tag & 6×His Tag) (A318369) in a linear range of 2.56-320 µg/ml.
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