The protein has a predicted molecular mass of 27.0 kDa after removal of the signal peptide. The apparent molecular mass of His-IGFBP7 is approximately 35-40 kDa due to glycosylation.
Conjugate
Unconjugated
Purity
> 85% by SDS-PAGE and Coomassie blue staining.
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Flow Cytometry - Recombinant Human IGFBP7 Protein (6xHis Tag) (A318064)
Flow cytometry analysis of 15µg/ml of Recombinant Human IGFBP7 Protein (6×His Tag) (A318064) on Expi293 cells transfected with Human CD93 protein (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
Recombinant Human IGFBP7 Protein (6xHis Tag) (A318064)
ELISA plates were pre-coated with Recombinant Human IGFBP7 Protein (6×His Tag) (A318064) at 2 µg/ml (100 µl/well) which can bind Anti-IGFBP7 Antibody [DMC422] - BSA and Azide free (A318750) in a linear range of 3.20-80 ng/ml.
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