Recombinant Human Mesothelin Protein (Fc Chimera 6xHis Tag)
Applications
ELISA, SDS-PAGE
Expression System
HEK293 cells
Nature
Recombinant
Protein Species
Human
Protein Length
Protein fragment.
Sequence
Mesothelin (Glu296-Gly580) + mFc (Pro99-Lys330) + 6xHis Tag
Tag
C-terminal Mouse Fc Tag and 6xHis Tag
Molecular Weight
The protein has a predicted molecular mass of 59.3 kDa after removal of the signal peptide.
Conjugate
Unconjugated
Purity
> 95% by SDS-PAGE and Coomassie blue staining.
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Recombinant Human Mesothelin Protein (Fc Chimera 6xHis Tag) (A318377)
ELISA plates were pre-coated with Recombinant Human Mesothelin Protein (Fc Chimera 6xHis Tag) (A318377) at 2 µg/ml (100 µl/well) which can bind Amatuximab Biosimilar - Anti-Mesothelin Antibody - BSA and Azide free (A318935) in a linear range of 3.2-80.0 ng/ml.
Recombinant Human Mesothelin Protein (Fc Chimera 6xHis Tag) (A318377)
ELISA plates were pre-coated with Recombinant Human Mesothelin Protein (Fc Chimera 6xHis Tag) (A318377) at 2 µg/ml (100 µl/well) which can bind LB1902 Biosimilar - Anti-Mesothelin Antibody - BSA and Azide free (A318810) in a linear range of 0.64-16 ng/ml.
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