The protein has a predicted molecular mass of 23.4 kDa after removal of the signal peptide. The apparent molecular mass of PDL2-His is approximately 35-55 kDa due to glycosylation.
Conjugate
Unconjugated
Purity
> 95% by SDS-PAGE and Coomassie blue staining.
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Flow Cytometry - Recombinant Human PD-L2 Protein (6xHis Tag) (A318218)
Flow cytometry analysis of 15µg/ml of Recombinant Human PD-L2 Protein (6×His Tag) (A318218) on Expi293 cells transfected with human PD1 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
Recombinant Human PD-L2 Protein (6xHis Tag) (A318218)
ELISA plates were pre-coated with Recombinant Human PD-L2 Protein (6×His Tag) (A318218) at 2 µg/ml (100 µl/well) which can bind Anti-PD-L2 Antibody [DM169] - BSA and Azide free (A318540) in a linear range of 0.13-3.20 ng/ml.
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