Consists of amino acids Arg319-Phe541 of SARS-CoV-2 Spike Glycoprotein RBD (Beta B.1.351 Variant) containing the following mutations: K417N, E484K, N501Y. The human Fc tag consists of amino acids Glu99-Ala330.
Applications
ELISA, SDS-PAGE
Expression System
HEK293 cells
Nature
Recombinant
Protein Species
SARS-CoV-2
Protein Length
Protein fragment.
Sequence
S protein RBD (K417N, E484K,N501Y) (Arg319-Phe541) + hFc (Glu99-Ala330)
Tag
C-terminal Human Fc Tag
Molecular Weight
The protein has a predicted molecular mass of 51.3 kDa after removal of the signal peptide. The apparent molecular mass of RBD(K417N, E484K,N501Y)-hFc is approximately 55-70 kDa due to glycosylation.
Conjugate
Unconjugated
Purity
> 90% by SDS-PAGE and Coomassie blue staining.
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
ELISA plates were pre-coated with 2 µg/ml (100 µl/well) Recombinant SARS-CoV-2 Spike Glycoprotein RBD (Beta B.1.351 Variant) (Fc Tag) (A318199) which can bind Recombinant Human ACE2 Protein (Fc Tag) (A318341) in a linear range of 12.346-3000 µg/ml.
ELISA plates were pre-coated with 2 µg/ml (100 µl/well) Recombinant SARS-CoV-2 Spike Glycoprotein RBD (Beta B.1.351 Variant) (Fc Tag) (A318199) which can bind Anti-SARS-CoV-2 Spike Glycoprotein RBD Antibody [DM55] - Azide free (A317410) in a linear range of 0.244-15.625 µg/ml.
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