Rabbit polyclonal antibody to Retinoblastoma (Phospho-Thr826)
Specificity
The antibody detects endogenous levels of Retinoblastoma only when phosphorylated at threonine 826.
Applications
WB, ICC/IF
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of threonine 826 (K-M-T(p)-P-R) derived from Human Retinoblastoma.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Western blot analysis of extracts from HepG2 cells, treated with nocodazole (1ug/ml, 16hours), using Retinoblastoma (Phospho-Thr826) antibody #12106. The lane on the right is treated with the synthesized peptide.
Immunofluorescence analysis of COS7 cells, using Retinoblastoma (Phospho-Thr826) antibody #12106. The picture on the right is treated with the synthesized peptide.
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