Anti-SAM Antibody [84-3] (A51746)

Immunofluorescence (IF) LSCM results of normal liver cells L02 cultured in RPMI 1640 with 10% FBS for 40h (A1-A4) double stained with Anti-SAH Antibody (AF488) (#MAF00302) at 40 µg/ml and MAF00202 at 40 µg/ml followed by DAPI staining. Hepatocellular carcinoma cell line HepG2 cells were cultured for 48h (B1-B4) and double stained with MAF00302 at 60 µg/ml and MAF00202 at 60 µg/ml followed by DAPI staining. Photography was performed under the laser scanning confocal microscope Zeiss LSM 780 (x630). Different views are as follows: DAPI (A1, B1), AF488 for SAH (A2, B2), R-PE for SAM (A3, B3); Overlap of all the three fluorescent signals (A4, B4). Expression patterns of SAM and SAH are different between L02 and HepG2 cells. In this case, both SAM and SAH are seen more in cytoplasm (more in mitochondria areas) than nuclear.

Flow Cytometry of L02 (B) and HepG2 (C) cells double stained with MAF00302 at 45 µg/ml and MAF00202 at 45 µg/ml. 100% confluent cells (cultured in RPMI 1640 with 10% FBS for 40h) were fixed and permeabilised with the nuclear fixation / permeabilisation buffer (eBioscience 00-5523 FoxP3_TF Staining Buffer Set) and then double stained with the antibodies indicated above. Cells were used for analysis with BD FACS Canto II Flow Cytometer. Both SAM and SAH are expressed ubiquitously yet rather dynamically. A: blank.