Western blot analysis of extracts of various cell lines, using SGTA antibody (A10109) at 1:1,000 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (A17345) at 1:10,000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Western blot analysis of extracts from normal (control) and SGTA knockout (KO) 293T cells, using SGTA antibody (A10109) at 1:1,000 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (A17345) at 1:10,000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.