SHP-1 (phospho Tyr564) Cell Based ELISA Kit (A103802)

SHP-1 (phospho Tyr564) Cell Based ELISA Kit

Cell-based (qualitative)

This SHP-1 (phospho Tyr564) Cell Based ELISA Kit allows for the detection of SHP-1 (phospho Tyr564) and the effects that certain stimulation conditions have on SHP-1 (phospho Tyr564) expression in different cell lines. Qualitative determination of SHP-1 (phospho Tyr564) concentration is achieved by an indirect ELISA format. In essence, SHP-1 (phospho Tyr564) is captured by Anti-SHP-1 (phospho Tyr564) Antibodies while Dye 1-conjugated and Dye 2-conjugated secondary antibodies bind the Fc region of the primary antibody. Through this binding, the dye conjugated to the secondary antibody can emit light at a certain wavelength given proper excitation, hence allowing for a fluorometric detection method. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target RFU values. Anti-SHP-1 Antibody is also included for normalization purposes. The RFU values obtained for the non-phosphorylated target can be used to normalize the RFU value for the phosphorylated target.

Fluorometric

> 5000 Cells

Human

Store at + 4°C. Stable for 6 months.

HCP, Hematopoietic cell protein-tyrosine phosphatase, Protein-tyrosine phosphatase 1C, Protein-tyrosine phosphatase SHP-1, PTP-1C, PTP1C, PTPN6, SH-PTP1, Tyrosine-protein phosphatase non-receptor type 6

Information online is representative. Always refer to the Product Manual inside the kit.