This SNAI1 (phospho Ser246) Cell Based ELISA Kit allows for the detection of SNAI1 (phospho Ser246) and the effects that certain stimulation conditions have on SNAI1 (phospho Ser246) expression in different cell lines. Qualitative determination of SNAI1 (phospho Ser246) concentration is achieved by an indirect ELISA format. In essence, SNAI1 (phospho Ser246) is captured by Anti-SNAI1 (phospho Ser246) Antibodies while Dye 1-conjugated and Dye 2-conjugated secondary antibodies bind the Fc region of the primary antibody. Through this binding, the dye conjugated to the secondary antibody can emit light at a certain wavelength given proper excitation, hence allowing for a fluorometric detection method. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target RFU values. Anti-SNAI1 Antibody is also included for normalization purposes. The RFU values obtained for the non-phosphorylated target can be used to normalize the RFU value for the phosphorylated target.
Platform
Microplate
Detection Type
Fluorometric
Product Range
> 5000 Cells
Reactivity
Human, Mouse
Storage
Store at + 4°C. Stable for 6 months.
Synonyms
Protein sna, Protein snail homolog 1, SNAH, Zinc finger protein SNAI1
Product Note
Information online is representative. Always refer to the Product Manual inside the kit.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - SNAI1 (phospho Ser246) Cell Based ELISA Kit (A103805)
The mouse monoclonal antibody to GAPDH is used as a positive control in SNAI1 (phospho Ser246) Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
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