Western blot analysis of extracts of various cell lines, using Anti-SPAM1 Antibody (A11511) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded rat testis using Anti-SPAM1 Antibody (A11511) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence analysis of rat testis cells using Anti-SPAM1 Antibody (A11511) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of human testis cells using Anti-SPAM1 Antibody (A11511) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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Alternative products to Anti-SPAM1 Antibody (A11511)
Recombinantmonoclonal antibody to SPAM1 for use as a research grade Berahyaluronidase Alfa biosimila for ELISA, Functional Studies and in vivo Research.
Recombinantmonoclonal antibody to SPAM1 for use as a research grade Berahyaluronidase Alfa biosimila for ELISA, Functional Studies and in vivo Research.
Proteins predicted to interact with SPAM1
Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.