Rabbit polyclonal antibody to Src (Phospho-Tyr418)
Specificity
The antibody detects endogenous level of Src only when phosphorylated at tyrosine 418.
Applications
WB, IHC
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of tyrosine 418(N-E-Y(p)-T-A)derived from Human Src.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Predicted MW
60kd
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Western blot analysis of extracts from COLO205 cells using Src (Ab-418) antibody (#21115, Lane 1 and 2) and Src (phospho-Tyr418) antibody (#11091, Lane 3 and 4).
