Rabbit polyclonal antibody to STAT1 (Phospho-Ser727)
Specificity
The antibody detects endogenous level of STAT1 only when phosphorylated at serine 727.
Applications
WB, IHC, ICC/IF
Reactivity
Human, Mouse
Immunogen
Peptide sequence around phosphorylation site of serine 727 (P-M-S(p)-P-E) derived from Human STAT1.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
Signal transducer and activator of transcription 1-alpha/beta, Transcription factor ISGF-3 components p91/p84
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using STAT1 (Phospho-Ser727) Antibody #11163 (left) or the same antibody preincubated with blocking peptide (right).