This Superoxide Anion Assay Kit is a quantitative assay kit for measuring Superoxide Anion in serum, plasma, animal and plant tissues, cells, and cell supernatant.
Applications
Functional Studies
Assay Type
Quantitative
Principle of Assay
Superoxide reacts with hydroxylamine hydrochloride to generate nitrite, which then forms an azo dye with Griess reagents. The absorbance at 540 nm is used to calculate superoxide anion content.
Platform
96-Well Microplate, Microcuvette
Sample Type
Serum, plasma, animal tissues, plant tissues, cells, and culture supernatant
Assay Time
30-60 minutes
Components
Extraction Buffer, Reagent I, Reagent II, Reagent III, NaNO2 Standard (10 mmoL/L)
Storage
Shipped at 4°C. Store at 4°C in the dark. Refer to the Protocol Manual for storage conditions of individual components. Do not use past expiration date!
General Notes
A 96-well microplate or microglass cuvette is required for this assay.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
