Principle of Assay
This kit provides a simple and easy colorimetric assay for the study of superoxide anion scavenging ability in animal and plant tissues, cells, and other biological fluids. In this assay, superoxide anion (O2-) is generated by xanthine oxidase catalyzed reaction. O2- reacts with a tetrazolium salt (WST-8) dye to form a water-soluble colored formazan product, which can be easily quantified. Superoxide scavengers in the sample reduce the levels of O2- thus less O2- is available for the chromogenic reaction. This scavenging ability of the sample is determined by measuring the absorbance produced at 450 nm.
