Synthetic nanodiscs offer a stable and biologically relevant environment that closely mimics cell membranes and enables full-length transmembrane human GPR84 protein to be purified and analysed in vitro.
Expression System
HEK293 cells
Nature
Synthetic
Protein Species
Human
Protein Length
Full length protein.
Tag
C-terminal Flag Tag
Molecular Weight
Full length human GPR84 protein has a MW of 43.7 kDa.
Conjugate
Unconjugated
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from nanodisc solubilization buffer (20mM Tris-HCl, 150mM NaCl, pH 8.0). Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
ELISA plates were pre-coated with Synthetic Nanodisc Human GPR84 Protein (A317301) at 0.2µg/per well. Serial diluted Anti-Flag Monoclonal Antibody solutions were added, washed, and incubated with a secondary antibody before reading the ELISA. From the above data, the EC50 for Anti-Flag Monoclonal Antibody binding with Synthetic Nanodisc Human GPR84 Protein (A317301) is 2.893ng/ml.
Western Blot - Synthetic Nanodisc Human GPR84 Protein (A317301)
Western Blot analysis of Synthetic Nanodisc Human GPR84 Protein (A317301) with Anti-Flag Monoclonal Antibody at 1/5000 dilution, followed by Goat Anti-Rabbit IgG HRP at 1/5000 dilution.
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