Principle of Assay
This kit provides a simple method for detecting activity of thioredoxin reductase (TrxR) in a variety of biological samples such as serum, plasma, animal tissues, cells, and bacteria. TrxR can catalyzes the reduction of DTNB by NADPH to generate TNB and NADP+. TNB has a characteristic absorption peak at 412 nm. However, reduced glutathione can also react with DTNB to form TNB, so this kit uses 2-vinylpyridine to block the reduced glutathione present in samples. TrxR activity can be calculated by measuring the rate of TNB formation at 412 nm.
