Anti-Thioredoxin / TRX Antibody (A88106)

Name: Anti-Thioredoxin / TRX Antibody
Brand: Antibodies.com
SKU: A88106

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This product is discontinued

Anti-Thioredoxin / TRX Antibody (A88106) has been discontinued and is no longer available.

View all Primary Antibodies.

Specifications

Name

Anti-Thioredoxin / TRX Antibody

Description

Rabbit polyclonal antibody to Thioredoxin / TRX.

Applications

WB, ICC/IF

Dilutions

WB: 1:100-1:500, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Thioredoxin 1 (Trx1/Thioredoxin 1 (Trx1/TXN)) (NP_003320.2).

Sequence

MVKQIESKTAFQEALDAAGDKLVVVDFSATWCGPCKMIKPFFHSLSEKYSNVIFLEVDVDDCQDVASECEVKCMPTFQFFKKGQKVGEFSGANKEKLEAT

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

12 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

ADF, ATL derived factor, ATL-derived factor, DKFZp686B1993, MGC61975, SASP, Surface associated sulphydryl protein, Surface-associated sulphydryl protein, testicular tissue protein Li 199, Thioredoxin, thioredoxin delta 3, THIO_HUMAN, TRDX, Trx, TRX 1, TRX1, TXN, TXN delta 3, TXN protein, zgc:92903

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-Thioredoxin / TRX Antibody (A88106)

Western blot analysis of extracts of various cell lines, using Anti-Thioredoxin / TRX Antibody (A88106) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Western Blot - Anti-Thioredoxin / TRX Antibody (A88106)

Western blot analysis of extracts of Rat liver, using Anti-Thioredoxin / TRX Antibody (A88106) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Immunofluorescence - Anti-Thioredoxin / TRX Antibody (A88106)

Immunofluorescence analysis of HeLa cells using Anti-Thioredoxin / TRX Antibody (A88106) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Immunofluorescence - Anti-Thioredoxin / TRX Antibody (A88106)

Immunofluorescence analysis of NIH-3T3 cells using Anti-Thioredoxin / TRX Antibody (A88106) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).