Principle of Assay
Tissue Primer is a universal enzyme blocking reagent to use in horse radish peroxidase (HRP) and alkaline phosphatase (AP) based immunohistochemical staining procedures done on paraffin tissue sections, frozen tissue sections and cell smears to suppress non-specific staining due to endogenous enzyme activity. In the evaluation of tissue specimens by IHC using HRP and AP methods, the presence of endogenous enzymes can often obscure the specific staining of the target antigen. Endogenous peroxidase and AP activity can be inhibited by the use of Tissue Primer which will suppress endogenous peroxidase, pseudoperoxidase, and alkaline phosphatase in paraffin tissue sections, frozen tissue sections and cell smears.
