This Total Iron-Binding Capacity Assay Kit is a quantitative assay kit for measuring Total Iron Binding Capacity in serum.
Applications
Functional Studies
Assay Type
Quantitative
Principle of Assay
Iron-binding capacity is determined by measuring iron complex formation with ferrozine before and after release of transferrin-bound iron. The difference in absorbance values corresponds to TIBC.
Platform
96-Well Microplate, Microcuvette
Sample Type
Serum
Assay Time
30-60 minutes
Components
Reagent I, Reagent II, Reagent III (A), Reagent III (B), Reagent IV, Standard
Storage
Shipped at 4°C. Refer to the Protocol Manual for storage conditions of individual components. Do not use past expiration date!
General Notes
A 96-well microplate or microglass cuvette is required for this assay.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Standard Curve - Total Iron-Binding Capacity Assay Kit (A319751)
Representative standard curve for Total Iron-Binding Capacity Assay Kit (A319751). The x-axis is total iron-binding capacity concentration (µmol/mL) and the y-axis is OD562.
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