Anti-TRIM Antibody [TRIM-04] (PE) (A86679)

Name: Anti-TRIM Antibody [TRIM-04] (PE)
Brand: Antibodies.com
SKU: A86679
Price: 360.0 GBP
Availability: InStock

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$585

Mouse monoclonal [TRIM-04] antibody to TRIM (PE) for Flow Cytometry.

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Alternative Formats and ConjugatesAlternative Formats

Unconjugated

Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-TRIM Antibody [TRIM-04] (PE)

Description

Mouse monoclonal [TRIM-04] antibody to TRIM (PE).

Specificity

This antibody reacts with T cell receptor-interacting molecule (TRIM), a 30 kDa adaptor protein expressed by T cells.

Applications

Flow Cytometry

Dilutions

Flow Cytometry: 1-4 µg/ml; Intracellular staining.

Reactivity

Human

Cross Reactivity

This antibody does not cross react with Mouse.

Immunogen

Recombinant intracellular domain (aa 29-186) of human TRIM.

Host

Mouse

Clonality

Monoclonal

Clone ID

TRIM-04

Isotype

IgG2a

Conjugate

Excitation: 565nm, Emission: 578nm

Purification

This antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.

Concentration

100 µg/ml

Predicted MW

30 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.

Storage

Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!

Synonyms

pp29/30, T-cell receptor-associated transmembrane adapter 1, T-cell receptor-interacting molecule, TCRIM, TRAT1

Isotype Controls

Mouse IgG2a [MOPC-173] (PE) (A86304)

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

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Flow Cytometry - Anti-TRIM Antibody [TRIM-04] (PE) (A86679)

Flow cytometry intracellular staining of human peripheral whole blood staining TRIM with Anti-TRIM Antibody [TRIM-04] (PE) (A86679), (concentration in sample 1 µg/ml).

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Flow Cytometry - Anti-TRIM Antibody [TRIM-04] (PE) (A86679)

Flow cytometry multicolor surface staining of human lymphocytes using Anti-CD3 Antibody [UCHT1] (APC) (A86518), (10 µl reagent / 100 µl of peripheral whole blood) and intracellular staining of human lymphocytes using Anti-TRIM Antibody [TRIM-04] (PE) (A86679), (concentration in sample 1 µg/ml).

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Flow Cytometry - Anti-TRIM Antibody [TRIM-04] (PE) (A86679)

Comparison of using Anti-TRIM Antibody [TRIM-04] (PE) (A86679) nonspecific intracellular staining (red) and Mouse IgG2a kappa Isotype Control [MOPC-173] (PE) (A86304) nonspecific intracellular staining (concentration in sample 5 µg/ml, same as anti-TRIM PE concentration, blue) co-stained by Anti-CD3 epsilon Antibody [145-2C11] (APC) (A86498), (concentration in sample 1,2 µg/ml) in flow cytometry analysis of murine splenocyte suspension. Clone TRIM-04 is not specific for murine TRIM.

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Flow Cytometry - Anti-TRIM Antibody [TRIM-04] (PE) (A86679)

Separation of TRIM positive CD3 positive human lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained with Anti-TRIM Antibody [TRIM-04] (PE) (A86679), (concentration in sample 1 µg/ml).