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Universal IP/Co-IP ToolKit (Magnetic Beads) (A319775)

Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.
Name
Universal IP/Co-IP ToolKit (Magnetic Beads)
Description
This toolkit provides magnetic bead-based reagents for efficient immunoprecipitation (IP) and co-immunoprecipitation (Co-IP). It enables convenient isolation of target proteins and protein complexes from biological samples.
Applications
IP, Co-IP. Functional Studies, WB, Protein Purification
Principle of Assay
Designed for immunoprecipitation (IP) and co-immunoprecipitation (Co-IP) experiments. The kit includes optimized native and denatured lysis buffers, Protein A/G magnetic beads for efficient capture of antibody–antigen complexes, and an IP negative control to monitor assay specificity. HRP-conjugated secondary antibodies specific for mouse IgG light chain and rabbit IgG light chain are provided to minimize heavy chain interference during Western blot detection.
Sample Type
Cell and tissue lysates
Assay Time
3-5 hours
Components
Non-Denaturing Lysis Buffer (25ml), Denaturing Lysis Buffer (25ml), Wash Buffer (20X) (20ml), Protein A/G Magnetic Beads (0.45ml), Elution Buffer (2ml), Neutralization Buffer (0.2ml), Proteinase Inhibitor Cocktail (100X) (0.2ml), Mouse IgG (1mg/ml) (30µl), Rabbit IgG (1mg/mL) (30µl), Goat Anti-Mouse IgG Light Chain Antibody (HRP) (30µl), Mouse Anti-Rabbit IgG Light Chain Antibody (HRP) (30µl)
Storage
Shipped at 4°C. Refer to the Protocol Manual for storage conditions of individual components. Do not use past expiration date!
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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