Primary Antibodies
Secondary Antibodies
Proteins & Peptides
ELISA Kits
About Us
Contact Us
Antibodies.com Logo Antibodies.com Logo
Sign In/Register
0
ISO 9001:2015 Certified
Live Customer Support
4.5/5 on Trustpilot
100% Quality Guarantee

Universal IP/Co-IP ToolKit (Magnetic Beads/Anti-Mouse) (A319776)

Immunoprecipitation - Universal IP/Co-IP ToolKit (Magnetic Beads/Anti-Mouse) (A319776) - Antibodies.com
$260
Product Datasheet
100% Guarantee
Price Match Guarantee
Request a Quotation
Custom or Bulk Request

Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Universal IP/Co-IP ToolKit (Magnetic Beads/Anti-Mouse)
Description
This kit uses magnetic beads coupled with anti-mouse antibodies for efficient immunoprecipitation and co-immunoprecipitation. It is suitable for isolating proteins or protein complexes using mouse primary antibodies.
Applications
IP, Co-IP. Functional Studies, WB, Protein Purification
Principle of Assay
Designed for IP and Co-IP experiments using mouse primary antibodies. The kit includes optimized native and denatured lysis buffers, Protein A/G magnetic beads for isolation of antibody–antigen complexes, and an IP negative control. An HRP-labeled goat anti-mouse IgG light chain secondary antibody is included to reduce heavy chain interference during Western blot analysis.
Sample Type
Cell and tissue lysates
Assay Time
3-5 hours
Components
Non-Denaturing Lysis Buffer (25ml), Wash Buffer (20X) (20ml), Protein A/G Magnetic Beads (0.5ml), Elution Buffer (2ml), Neutralization Buffer (0.2ml), Proteinase Inhibitor Cocktail (100X) (0.2ml), Mouse IgG (1mg/ml) (30µl), Goat Anti-Mouse IgG Light Chain Antibody (HRP) (30µl)
Storage
Shipped at 4°C. Refer to the Protocol Manual for storage conditions of individual components. Do not use past expiration date!
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (1)

Immunoprecipitation - Universal IP/Co-IP ToolKit (Magnetic Beads/Anti-Mouse) (A319776) - Antibodies.comEnlarge Image

Immunoprecipitation - Universal IP/Co-IP ToolKit (Magnetic Beads/Anti-Mouse) (A319776)

Co-IP performed using Universal IP/Co-IP Toolkit (Magnetic Beads/Anti-Mouse) (A319776). Protein was extracted using non-denaturing lysis buffer, and then analyzed by Co-IP. The whole cell lysates (Input) and Co-IP samples (using the indicated antibodies) were probed with Stat1 monoclonal antibody, Stat2 polyclonal antibody, and GAPDH monoclonal antibody respectively in Western blot analysis.
Publishing research using Universal IP/Co-IP ToolKit (Magnetic Beads/Anti-Mouse) (A319776)? Please let us know so that we can list the citation on this page.