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Universal IP/Co-IP ToolKit (Magnetic Beads/Anti-Rabbit) (A319777)

Immunoprecipitation - Universal IP/Co-IP ToolKit (Magnetic Beads/Anti-Rabbit) (A319777) - Antibodies.com
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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Universal IP/Co-IP ToolKit (Magnetic Beads/Anti-Rabbit)
Applications
IP
Principle of Assay
Designed for IP/Co-IP experiments. This product contains: optimized native and denatured lysate, Protein A/G magnetic beads, IP negative control, and an optimized secondary antibody (HRP-labeled mouse anti-rabbit light chain) to eliminate heavy chain interference.
Sample Type
Animal and plant tissues, cells, and bacteria.
Components
Non-Denaturing Lysis Buffer (25ml), Wash Buffer (20X) (20ml), Protein A/G Magnetic Beads (0.5ml), Elution Buffer (2ml), Neutralization Buffer (0.2ml), Proteinase Inhibitor Cocktail (100X) (0.2ml), Rabbit IgG (1mg/ml) (30µl), Mouse Anti-Rabbit IgG Light Chain Anitbody (HRP) (30µl)
Storage
Shipped at 4°C. Refer to the Protocol Manual for storage conditions of individual components. Do not use past expiration date!
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (1)

Immunoprecipitation - Universal IP/Co-IP ToolKit (Magnetic Beads/Anti-Rabbit) (A319777) - Antibodies.comEnlarge Image

Immunoprecipitation - Universal IP/Co-IP ToolKit (Magnetic Beads/Anti-Rabbit) (A319777)

Co-IP performed using Universal IP/Co-IP Toolkit (Magnetic Beads/Anti-Rabbit) (A319777). Protein was extracted using non-denaturing lysis buffer, and then analyzed by Co-IP. The whole cell lysates (Input) and Co-IP samples (using the indicated antibodies) were probed with Stat1 monoclonal antibody, Stat2 polyclonal antibody, and GAPDH monoclonal antibody respectively in Western blot analysis.
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