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Anti-V5-Tag Antibody (A50030)

V5-Tag Mouse Monoclonal Antibody from Signalway Antibody (T605) - Antibodies.com V5-Tag Mouse Monoclonal Antibody from Signalway Antibody (T605) - Antibodies.com V5-Tag Mouse Monoclonal Antibody from Signalway Antibody (T605) - Antibodies.com
$290
Product Datasheet

Mouse monoclonal antibody to V5-Tag for ELISA, WB, ICC/IF, IHC and Flow Cytometry.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 6-9 business days.

  • Specifications

Name
Anti-V5-Tag Antibody
Description
Mouse monoclonal antibody to V5-Tag
Specificity
V5-tag mouse monoclonal antibody detects endogenous levels of internal, C-terminal, and N-terminal V5- tagged proteins.
Applications
ELISA, WB, ICC/IF, IHC, Flow Cytometry
Dilutions
Western blotting 1:3000-1:10000 Immunohistochemistry 1:200
Reactivity
Species Independent
Immunogen
A synthetic peptide GKPIPNPLLGLDST coupled to KLH.
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Conjugate

Unconjugated

Purification
Affinity-chromatography
Concentration
1.0 mg/mL
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Mouse IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (3)

V5-Tag Mouse Monoclonal Antibody from Signalway Antibody (T605) - Antibodies.comEnlarge Image

Anti-V5-Tag Antibody (A50030)

All lanes: V5-tag mouse monoclonal antibody HEK 293 cell lysate transfected without V5-tag protein B: HEK 293 cell lysate transfected with V5 -tag protein C: IP (HEK 293 cell without transfected + anti- V5 mAb 4℃ overnight) D: IP (PBS + anti- V5 mAb 4℃ overnight) E: IP (HEK293 transfected+protein G+anti- V5 mAb 4℃ overnight) F: IP (HEK293 transfected+RIPA+Protein G 4℃ overnight) G: Multi Tag Recombinant protein (expressed in E.coli) TP=Target Protein HC=Heavy chain LC=Light Chain
V5-Tag Mouse Monoclonal Antibody from Signalway Antibody (T605) - Antibodies.comEnlarge Image

Anti-V5-Tag Antibody (A50030)

IF analysis of 293 cells transfected with a V5-tag protein, using anti-V5Tag Mouse mAb at a 1: 2000 dilution (blue DAPI, red anti-V5)
V5-Tag Mouse Monoclonal Antibody from Signalway Antibody (T605) - Antibodies.comEnlarge Image

Anti-V5-Tag Antibody (A50030)

IP antibody use：5ug V5 Mouse IgG1 per ml 1: 5000 1) untransfected 293 cell lysate 2) transfected 293 cell lysate with V5-tag fusion protein3) IP (untransfected 293+anti-V5 mAb+Protein G agarose) 4) IP (transfected 293+ normal Mouse IgG+Protein G agarose) 5) IP (transfected 293+anti-V5 mAb+ Protein G agarose) 6) IP (transfected 293+Protein G) 7) Recombinant protein (E.coli)
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