Rabbit polyclonal antibody to Vitamin D3 Receptor (Phospho-Ser51)
Specificity
The antibody detects endogenous levels of Vitamin D3 Receptor only when phosphorylated at serine 51.
Applications
WB
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of serine 51 (R-R-S(p)-M-K) derived from Human Vitamin D3 Receptor.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
1,25-dihydroxyvitamin D3 receptor, NR1I1, Nuclear receptor subfamily 1 group I member 1
Western blot analysis of extracts from COLO cells, treated with Insulin (0.01U/ml, 15mins), using Vitamin D3 Receptor (Phospho-Ser51) antibody #12155. The lane on the right is treated with the synthesized peptide.
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