ZNRF1 expression in N2a cell lysate and Mouse Brain lysates (Embryo E14 and adult cerebellum) analyzed by western blot. Cells were transfected to overexpress ZNRF1 and incubated with Anti-ZNRF1 Antibody (A82537) at 1µg/ml and detcted by chemiluminescence.
ZNRF1 expression in Mouse Cerebral Cortex analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by microwaving in citrate buffer, pH 6. Staining was performed with Anti-ZNRF1 Antibody (A82537) at 10µg/ml and revealed with a biotinylated Anti-goat secondary antibody and streptavidin-conjugated Alexa 488. Note the Neurofilament M was labeled by Millipore at a dilution of 1:100.
ZNRF1 expression in Human Cerebellum analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-ZNRF1 Antibody (A82537) at 5µg/ml and revealed with alkaline phosphatase (AP).
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Proteins predicted to interact with ZNRF1
Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.