Anti-CNPase Antibody [ARC0388] (A308067)

Name: Anti-CNPase Antibody [ARC0388]
Brand: Antibodies.com
SKU: A308067

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This product is discontinued

Anti-CNPase Antibody [ARC0388] (A308067) has been discontinued and is no longer available.

View all Anti-CNPase Antibodies.

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Specifications

Name

Anti-CNPase Antibody [ARC0388]

Description

Rabbit monoclonal [ARC0388] antibody to CNPase.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 322-421 of human CNPase (P09543).

Sequence

NLPRGSRAHITLGCAADVEAVQTGLDLLEILRQEKGGSRGEEVGELSRGKLYSLGNGRWMLTLAKNMEVRAIFTGYYGKGKPVPTQGSRKGGALQSCTII

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0388

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

48 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

2'', 2'3' cyclic nucleotide 3' phosphodiesterase, 3''-cyclic-nucleotide 3''-phosphodiesterase, CN37_HUMAN, CNP, CNP 1, CNP1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-CNPase Antibody [ARC0388] (A308067)

Western blot analysis of extracts of various cell lines, using Anti-CNPase Antibody [ARC0388] (A308067) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Immunohistochemistry - Anti-CNPase Antibody [ARC0388] (A308067)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-CNPase Antibody [ARC0388] (A308067) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-CNPase Antibody [ARC0388] (A308067)

Immunohistochemistry analysis of paraffin-embedded mouse cerebellum using Anti-CNPase Antibody [ARC0388] (A308067) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-CNPase Antibody [ARC0388] (A308067)

Immunohistochemistry analysis of paraffin-embedded human brain tissue using Anti-CNPase Antibody [ARC0388] (A308067) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.