Anti-Cofilin (phospho Ser3) Antibody (A16495)

Name: Anti-Cofilin (phospho Ser3) Antibody
Brand: Antibodies.com
SKU: A16495
Price: 435.0 USD
Availability: InStock

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$435

Rabbit polyclonal antibody to Cofilin (phospho Ser3) for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Anti-Cofilin (M1) Antibody

Rabbit polyclonal antibody to Cofilin (M1) for WB, IHC and IF.

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Anti-Cofilin (M1) Antibody

Rabbit polyclonal antibody to Cofilin (M1) for WB, IHC and IF.

Specifications

Name

Anti-Cofilin (phospho Ser3) Antibody

Description

Rabbit polyclonal antibody to Cofilin (phospho Ser3).

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:100, ICC/IF: 1:100-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around S3 of human CFL1 (NP_005498.1).

Sequence

MASGV

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

18 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

18 kDa phosphoprotein, CFL, CFL 1, CFL1, COF1_HUMAN, Cofilin 1, Cofilin 1 non muscle, Cofilin non muscle isoform, Cofilin-1, epididymis secretory protein Li 15, HEL-S-15, non-muscle isoform, p18

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-Cofilin (phospho Ser3) Antibody (A16495)

Western blot analysis of extracts of NIH/3T3 cells, using Anti-Cofilin (phospho Ser3) Antibody (A16495) at 1:1,000 dilution or CFL1 antibody (A1704). NIH/3T3 cells were treated by CIP(20uL/400ul) at 37°C for 1 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 3s.

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Figure 2: Immunohistochemistry - Anti-Cofilin (phospho Ser3) Antibody (A16495)

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Anti-Cofilin (phospho Ser3) Antibody (A16495). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.