Anti-COPB2 Antibody [ARC52625 + ARC52626] (A309336)

Name: Anti-COPB2 Antibody [ARC52625 + ARC52626]
Brand: Antibodies.com
SKU: A309336

Enlarge Image

This product is discontinued

Anti-COPB2 Antibody [ARC52625 + ARC52626] (A309336) has been discontinued and is no longer available.

View all Anti-COPB2 Antibodies.

Specifications

Name

Anti-COPB2 Antibody [ARC52625 + ARC52626]

Description

Rabbit Oligoclonal [ARC52625 + ARC52626] antibody to COPB2.

Applications

WB, ICC/IF

Dilutions

WB: 1:2,000-1:20,000, ICC/IF: 1:100-1:500

Reactivity

Human, Mouse

Immunogen

Recombinant protein of human COPB2.

Host

Rabbit

Clonality

Oligoclonal

Clone ID

ARC52625 + ARC52626

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

102 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

beta COP I, Beta'-coat protein, Beta'-COP, Coatomer subunit beta', COPB2_HUMAN, p102

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

Enlarge Image

Figure 1: Western Blot - Anti-COPB2 Antibody [ARC52625 + ARC52626] (A309336)

Western blot analysis of various lysates, using Anti-COPB2 Antibody [ARC52625 + ARC52626] (A309336) at 1:20,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

Enlarge Image

Figure 2: Western Blot - Anti-COPB2 Antibody [ARC52625 + ARC52626] (A309336)

Western blot analysis of extracts from wild type(WT) and COPB2 knockdown (KD) Hela(KD) cells, using Anti-COPB2 Antibody [ARC52625 + ARC52626] (A309336) at 1:20,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

Enlarge Image

Figure 3: Immunofluorescence - Anti-COPB2 Antibody [ARC52625 + ARC52626] (A309336)

Immunofluorescence analysis of HeLa using Anti-COPB2 Antibody [ARC52625 + ARC52626] (A309336) at a dilution of 1:500 (40x lens). DAPI was used to stain the cell nuclei (blue).

Publishing research using Anti-COPB2 Antibody [ARC52625 + ARC52626] (A309336)? Please let us know so that we can list the citation on this page.