Anti-COT (phospho Thr290) Antibody (A93785)

Name: Anti-COT (phospho Thr290) Antibody
Brand: Antibodies.com
SKU: A93785
Price: 185.0 USD
Availability: InStock

This product is identical to Abcam (ab51214). Discover more for less.

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$185

Rabbit polyclonal antibody to COT (phospho Thr290) for WB, IHC, IF and ELISA.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-COT (phospho Thr290) Antibody

Description

Rabbit polyclonal antibody to COT (phospho Thr290).

Specificity

This antibody detects endogenous levels of COT only when phosphorylated at Thr290.

Applications

WB, IHC, IF, ELISA

Dilutions

WB: 1:500-1:1000, IHC: 1:50-1:100, IF: 1:100-1:500, ELISA: 1:40000

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide derived from human COT around the phosphorylation site of Thr290 (amino acids 256-305).

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.

Molecular Weight

52kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

AURA2, c COT, Cancer Osaka thyroid oncogene, CCOT, COT, COT proto oncogene serine/threonine protein kinase, EST, ESTF, Ewing sarcoma transformant, FLJ10486, M3K8_HUMAN, MAP3K 8, MEKK8, Mitogen activated protein kinase kinase kinase 8, Mitogen-activated protein kinase kinase kinase 8, Proto oncogene cCot, Proto-oncogene c-Cot, Serine/threonine protein kinase cot, Serine/threonine-protein kinase cot, TPL 2, TPL-2, TPL2, Tumor progression locus 2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-COT (phospho Thr290) Antibody (A93785)

Western blot analysis of lysates from 293 cells treated with UV 15' using Anti-COT (phospho Thr290) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.

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Figure 2: Immunohistochemistry - Anti-COT (phospho Thr290) Antibody (A93785)

Immunohistochemical analysis of paraffin-embedded human brain using Anti-COT (phospho Thr290) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.

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Figure 3: Immunofluorescence - Anti-COT (phospho Thr290) Antibody (A93785)

Immunofluorescence analysis of HUVEC cells using Anti-COT (phospho Thr290) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.