Anti-COX IV Antibody (A309799)

Name: Anti-COX IV Antibody
Brand: Antibodies.com
SKU: A309799
Price: 470.0 USD
Availability: InStock

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$470

Rabbit polyclonal antibody to COX IV for WB and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Tags:

Mitochondrial Loading Control Mitochondrial Marker

Specifications

Name

Anti-COX IV Antibody

Description

Rabbit polyclonal antibody to COX IV.

Applications

WB, ICC/IF

Dilutions

WB: 1:100-1:500, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human COX IV.

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

17 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

AL024441, COX 4, COX IV 1, COX IV-1, Cox4, COX4-1, COX41_HUMAN, Cox4a, COX4B, COX4I1, COX4I2, COX4L2, COXIV, Cytochrome c oxidase polypeptide IV, Cytochrome c oxidase subunit 4 isoform 1, Cytochrome c oxidase subunit 4 isoform 1 mitochondrial, Cytochrome c oxidase subunit 4 isoform 1, mitochondrial, Cytochrome C Oxidase subunit IV, Cytochrome c oxidase subunit IV isoform 1, Cytochrome c oxidase subunit IV isoform 2 (lung), Cytochrome c oxydase subunit 4, dJ857M17.2, MGC105470, MGC72016, mitochondrial

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-COX IV Antibody (A309799)

Western blot analysis of various lysates, using Anti-COX IV Antibody (A309799) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Western Blot - Anti-COX IV Antibody (A309799)

Western blot analysis of extracts from wild type(WT) and COX IVknockdown (KD) 293T(KD) cells, using Anti-COX IV Antibody (A309799) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 3: Immunofluorescence - Anti-COX IV Antibody (A309799)

Immunofluorescence analysis of HeLa cells using Anti-COX IV Antibody (A309799) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 4: Immunofluorescence - Anti-COX IV Antibody (A309799)

Immunofluorescence analysis of HepG2 cells using Anti-COX IV Antibody (A309799) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).