Anti-CREB (phospho Ser133) Antibody (A10929)

Name: Anti-CREB (phospho Ser133) Antibody
Brand: Antibodies.com
SKU: A10929
Price: 435.0 USD
Availability: InStock

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$435

Rabbit polyclonal antibody to CREB (phospho Ser133) for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-CREB (phospho Ser133) Antibody

Description

Rabbit polyclonal antibody to CREB (phospho Ser133).

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:100

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around S133 of human CREB1 (NP_604391.1).

Sequence

RPSYR

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

40 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Active transcription factor CREB, cAMP response element binding protein, cAMP response element binding protein 1, cAMP responsive element binding protein 1, cAMP-responsive element-binding protein 1, CREB-1, CREB1, CREB1_HUMAN, Cyclic AMP-responsive element-binding protein 1, MGC9284, OTTHUMP00000163864, OTTHUMP00000163865, OTTHUMP00000206660, OTTHUMP00000206662, OTTHUMP00000206667, Transactivator protein

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-CREB (phospho Ser133) Antibody (A10929)

Western blot analysis of extracts of NIH/3T3 cells, using Anti-CREB (phospho Ser133) Antibody (A10929) at 1:1,000 dilution. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. NIH/3T3 cells were treated by Anisomycin (25 ug/ml) at 37°C for 30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 2: Immunohistochemistry - Anti-CREB (phospho Ser133) Antibody (A10929)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-CREB (phospho Ser133) Antibody (A10929) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-CREB (phospho Ser133) Antibody (A10929)

Immunohistochemistry analysis of paraffin-embedded human tonsil using Anti-CREB (phospho Ser133) Antibody (A10929) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-CREB (phospho Ser133) Antibody (A10929)

Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-CREB (phospho Ser133) Antibody (A10929) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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