Anti-CRM1 Antibody [ARC0104] (A306284)

Name: Anti-CRM1 Antibody [ARC0104]
Brand: Antibodies.com
SKU: A306284

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This product is discontinued

Anti-CRM1 Antibody [ARC0104] (A306284) has been discontinued and is no longer available.

View all Anti-CRM1 Antibodies.

Specifications

Name

Anti-CRM1 Antibody [ARC0104]

Description

Rabbit monoclonal [ARC0104] antibody to CRM1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 972-1071 of human CRM1/XPO1 (O14980).

Sequence

IFLQEYVANLLKSAFPHLQDAQVKLFVTGLFSLNQDIPAFKEHLRDFLVQIKEFAGEDTSDLFLEEREIALRQADEEKHKRQMSVPGIFNPHEIPEEMCD

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0104

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

110 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Chromosome region maintenance 1 protein homolog, CRM 1, CRM1 homolog, DKFZp686B1823, emb, Exp 1, Exp1, Exportin 1, Exportin-1, Exportin1, XPO 1, xpo1, XPO1_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (8)

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Figure 1: Western Blot - Anti-CRM1 Antibody [ARC0104] (A306284)

Western blot analysis of extracts of mouse lung, using Anti-CRM1 Antibody [ARC0104] (A306284) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.

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Figure 2: Western Blot - Anti-CRM1 Antibody [ARC0104] (A306284)

Western blot analysis of extracts of various cell lines, using Anti-CRM1 Antibody [ARC0104] (A306284) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 3: Immunohistochemistry - Anti-CRM1 Antibody [ARC0104] (A306284)

Immunohistochemistry analysis of paraffin-embedded rat spleen using Anti-CRM1 Antibody [ARC0104] (A306284) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-CRM1 Antibody [ARC0104] (A306284)

Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-CRM1 Antibody [ARC0104] (A306284) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.