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Anti-Cx50 Antibody (A308599)

Western Blot - Anti-Cx50 Antibody (A308599) - Antibodies.com Immunofluorescence - Anti-Cx50 Antibody (A308599) - Antibodies.com Immunofluorescence - Anti-Cx50 Antibody (A308599) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to Cx50 for WB and ICC/IF.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-Cx50 Antibody
Description
Rabbit polyclonal antibody to Cx50.
Applications
WB, ICC/IF
Dilutions
WB: 1:500-1:1,000, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 234-433 of human GJA8 (NP_005258.2).
Sequence
SALKRPVEQPLGEIPEKSLHSIAVSSIQKAKGYQLLEEEKIVSHYFPLTEVGMVETSPLPAKPFNQFEEKISTGPLGDLSRGYQETLPSYAQVGAQEVEGEGPPAEEGAEPEVGEKKEEAERLTTEEQEKVAVPEGEKVETPGVDKEGEKEEPQSEKVSKQGLPAEKTPSLCPELTTDDARPLSRLSKASSRARSDDLTV
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
48 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
CAE, CAE1, cataract, zonular pulverulent 1 (FY-linked), Connexin 50, Connexin-50, CXA8_HUMAN, Gap junction alpha 8 protein, Gap junction alpha-8 protein, Gap junction membrane channel protein alpha 8 (connexin 50), Gap junction protein alpha 8, gap junction protein, alpha 8, 50kD, GJA8, Lens fiber protein MP70
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Cx50 Antibody (A308599) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Cx50 Antibody (A308599)
Western blot analysis of extracts of Raji cells, using Anti-Cx50 Antibody (A308599) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunofluorescence - Anti-Cx50 Antibody (A308599) - Antibodies.comEnlarge Image
Figure 2: Immunofluorescence - Anti-Cx50 Antibody (A308599)
Immunofluorescence analysis of C6 cells using Anti-Cx50 Antibody (A308599) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-Cx50 Antibody (A308599) - Antibodies.comEnlarge Image
Figure 3: Immunofluorescence - Anti-Cx50 Antibody (A308599)
Immunofluorescence analysis of L929 cells using Anti-Cx50 Antibody (A308599) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
Publishing research using Anti-Cx50 Antibody (A308599)? Please let us know so that we can list the citation on this page.

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