Anti-CXCR2 Antibody (A9177)

Name: Anti-CXCR2 Antibody
Brand: Antibodies.com
SKU: A9177
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to CXCR2 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-CXCR2 Antibody

Description

Rabbit polyclonal antibody to CXCR2.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 260 to the C-terminus of human CXCR2 (NP_001548.1).

Sequence

FLLCWLPYNLVLLADTLMRTQVIQETCERRNHIDRALDATEILGILHSCLNPLIYAFIGQKFRHGLLKILAIHGLISKDSLPKDSRPSFVGSSSGHTSTTL

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

46 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

C-X-C chemokine receptor type 2, CD 182, CD182, CD182 antigen, CDw128b, Chemokine (CXC) receptor 2, CMKAR2, CXC-R2, CXCR 2, CXCR-2, CXCR2_HUMAN, GRO/MGSA receptor, High affinity interleukin-8 receptor B, IL 8 receptor type 2, IL 8R B, IL-8 receptor type 2, IL-8R B, IL8 RB, IL8 receptor type 2, IL8R B, IL8R2, IL8RA, Interleukin 8 Receptor B, Interleukin 8 receptor, beta, Interleukin 8 receptor, type 2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-CXCR2 Antibody (A9177)

Western blot analysis of extracts of various cell lines, using Anti-CXCR2 Antibody (A9177) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Western Blot - Anti-CXCR2 Antibody (A9177)

Western blot analysis of extracts of THP-1 cells, using Anti-CXCR2 Antibody (A9177) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 3: Immunohistochemistry - Anti-CXCR2 Antibody (A9177)

Immunohistochemistry analysis of paraffin-embedded mouse spleen using Anti-CXCR2 Antibody (A9177) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-CXCR2 Antibody (A9177)

Immunohistochemistry analysis of paraffin-embedded rat spleen using Anti-CXCR2 Antibody (A9177) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.