Anti-CXCR4 Antibody [12G5] (PE) (A278995)

$635

Mouse monoclonal [12G5] antibody to CXCR4 (PE) for Flow Cytometry.

Alternative Formats and ConjugatesAlternative Formats

Freight Charges
Lead Time
4-6 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
Anti-CXCR4 Antibody [12G5] (PE)
Description
Mouse monoclonal [12G5] antibody to CXCR4 (PE).
Specificity
This antibody recognises CD184 also known as C-X-C chemokine receptor 4 (CXCR4) and fusion which is a G-protein-coupled chemokine receptor. It has an extracellular N-terminus, a 7-transmembrane structure with 7 helical regions connected by 6 membrane loops and a cytoplasmic C-terminus. It is expressed in a number of hematopoietic cells including hematopoietic stem cells, T cells, B cells, monocytes, macrophages, neutrophils and eosinophils. CD184’s ligand is C-X-C chemokine ligand 12 (CXCL12) which is also known as stromal cell-derived factor 1 (SDF-1). Upon binding of the ligand signaling events are initiated which can result in alterations in gene expression, actin polymerisation, cell skeleton rearrangement and cell migration. Via these pathways CD184 plays a role in homing hematopoietic stem and progenitor cells in bone marrow and mobilizes the cells to the periphery during stress or injury. As well as homeostatic processes, CD184 has also been linked with various pathologies, including systemic lupus erythematosus, rheumatoid arthritis, multiple sclerosis and tumorogenesis. Its expression in CD4+ T cells has also shown that it acts as a natural co-receptor for HIV type I by assisting with triggering membrane fusion of the virus to the target cell. Clone 12G5 has been used in flow cytometry experiments to examine CD184 cell surface expression in cells modified to express ?20 IFNITM2, an IFN-induced transmembrane protein which is known for its ability to inhibit the binding of several human viruses.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 1x106 cells in 100µl
Reactivity
Human, Primate
Immunogen
CP-MAC-infected Sup-T1 cells.
Host
Mouse
Clonality
Monoclonal
Clone ID
12G5
Isotype
IgG2a
Conjugate

PE

Excitation: 565nm, Emission: 578nm

Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
Lot Specific
Purity
>95% (by SDS-PAGE).
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.2% BSA and <0.1% Sodium Azide.
Storage
Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light.
General Notes
Mouse anti Human CD184 clone 12G5, recognizes CD184 also known as C-X-C chemokine receptor 4 (CXCR4) and fusion which is a G-protein-coupled chemokine receptor. It has an extracellular N-terminus, a 7-transmembrane structure with 7 helical regions connected by 6 membrane loops and a cytoplasmic C-terminus. It is expressed in a number of hematopoietic cells including hematopoietic stem cells, T cells, B cells, monocytes, macrophages, neutrophils and eosinophils (Teicher & Fricker 2010). CD184’s ligand is C-X-C chemokine ligand 12 (CXCL12) which is also known as stromal cell-derived factor 1 (SDF-1). Upon binding of the ligand signaling events are initiated which can result in alterations in gene expression, actin polymerisation, cell skeleton rearrangement and cell migration (Domanska et al. 2013). Via these pathways CD184 plays a role in homing hematopoietic stem and progenitor cells in bone marrow and mobilizes the cells to the periphery during stress or injury (Pawig et al. 2015). As well as homeostatic processes, CD184 has also been linked with various pathologies, including systemic lupus erythematosus, rheumatoid arthritis, multiple sclerosis and tumorogenesis. Its expression in CD4+ T cells has also shown that it acts as a natural co-receptor for HIV type I by assisting with triggering membrane fusion of the virus to the target cell (Feng et al. 1996). Clone 12G5 has been used in flow cytometry experiments to examine CD184 cell surface expression in cells modified to express ?20 IFNITM2, an IFN-induced transmembrane protein which is known for its ability to inhibit the binding of several human viruses (Wu et al. 2017).
Synonyms
C-X-C chemokine receptor type 4, CD184, CD184 antigen, Chemokine (C X C motif) receptor 4, Chemokine CXC Motif Receptor 4, CXC-R4, CXCR-4, CXCR4_HUMAN, D2S201E, FB22, Fusin, HM89, HSY3RR, LAP 3, LAP3, LCR1, LESTR, Leukocyte derived seven transmembrane domain receptor, Leukocyte-derived seven transmembrane domain receptor, Lipopolysaccharide associated protein 3, Neuropeptide Y receptor Y3, NPY3R, NPYR, NPYRL, NPYY3, NPYY3R, Probable G protein coupled receptor lcr1 homolog, SDF 1 receptor, SDF-1 receptor, SEVEN-TRANSMEMBRANE-SEGMENT RECEPTOR, Stromal cell derived factor 1 receptor, Stromal cell-derived factor 1 receptor, WHIM, WHIMS
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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