Anti-Cyclin B1 Antibody [ARC2614] (A309196)

Name: Anti-Cyclin B1 Antibody [ARC2614]
Brand: Antibodies.com
SKU: A309196
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC2614] antibody to Cyclin B1 for WB, IHC and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-Cyclin B1 Antibody [ARC2614]

Description

Rabbit monoclonal [ARC2614] antibody to Cyclin B1.

Applications

WB, IHC, IP

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 334-433 of human Cyclin B1 (P14635).

Sequence

YDMVHFPPSQIAAGAFCLALKILDNGEWTPTLQHYLSYTEESLLPVMQHLAKNVVMVNQGLTKHMTVKNKYATSKHAKISTLPQLNSALVQDLAKAVAKV

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC2614

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

55 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CCNB, CCNB 1, ccnb1, CCNB1_HUMAN, G2 mitotic specific cyclin B1, G2/mitotic-specific cyclin-B1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-Cyclin B1 Antibody [ARC2614] (A309196)

Western blot analysis of Jurkat, using Anti-Cyclin B1 Antibody [ARC2614] (A309196) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Immunohistochemistry - Anti-Cyclin B1 Antibody [ARC2614] (A309196)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-Cyclin B1 Antibody [ARC2614] (A309196) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Western Blot - Anti-Cyclin B1 Antibody [ARC2614] (A309196)

Immunoprecipitation analysis of 300µg extracts of HeLa cells using 3µg of Anti-Cyclin B1 Antibody [ARC2614] (A309196). This Western blot was performed on the immunoprecipitate using Anti-Cyclin B1 Antibody [ARC2614] (A309196) at a dilution of 1:1000.

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