Recombinant Anti-Cyclin B1 Antibody [CCNB1/7030R] (A278086)

Anti-Cyclin B1 Antibody [CCNB1/7030R]

Recombinant rabbit monoclonal [CCNB1/7030R] antibody to Cyclin B1.

It recognizes a protein of 55-62kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition.

Human, Mouse, Hamster

Recombinant full-length human Cyclin B1 protein.

Rabbit

Monoclonal

CCNB1/7030R

IgG

kappa

Unconjugated

200 µg/ml

55-62 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-Cyclin B1 Antibody [CCNB1/7030R] - BSA and Azide free (A278674).

CCNB, CCNB 1, ccnb1, CCNB1_HUMAN, G2 mitotic specific cyclin B1, G2/mitotic-specific cyclin-B1