Mouse monoclonal [V152] antibody to Cyclin B1 (PE).
Specificity
This antibody recognizes cyclin B1, a 48 kDa intracellular protein necessary for G2/M phase transition of the cell cycle.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests; Intracellular staining.
Reactivity
Mammalian
Immunogen
Recombinant His-tagged hamster cyclin B1.
Host
Mouse
Clonality
Monoclonal
Clone ID
V152
Isotype
IgG1
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
This antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Predicted MW
48 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Separation of K562 cells stained with Anti-Cyclin B1 Antibody [V152] (PE) (10 µl reagent per million cells in 100 µl of cell suspension, red-filled) from K562 cells stained with Mouse IgG1 [MOPC-21] (APC) - Isotype Control (concentration in sample is 6 µg/ml, same as Anti-Cyclin B1 Antibody [V152] (PE) concentration, black-dashed) in flow cytometry analysis (intracellular staining) of K562 cell suspension.
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