Rabbit polyclonal antibody to Cyclin C (Phospho-Ser275)
Specificity
The antibody detects endogenous levels of Cyclin C only when phosphorylated at serine 275.
Applications
WB, IHC
Dilutions
Western blotting: 1:500~1:1000Immunohistochemistry: 1:50~1:100
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of Serine 275(N-G-S(p)-A-N) derived from Human Cyclin C.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0mg / ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Figure 1: Anti-Cyclin C (Phospho-Ser275) Antibody (A50387)
Western blot analysis of extracts from 3T3 cells (Lane 2), A549 cells (Lane 3) and Hela cells (Lane 4), using Cyclin C (Phospho-Ser275) Antibody #11797. The lane on the left is treated with antigen-specific peptide.
Figure 2: Immunohistochemistry - Anti-Cyclin C (Phospho-Ser275) Antibody (A50387)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Cyclin C (Phospho-Ser275) antibody #11797 (left)or the same antibody preincubated with blocking peptide (right).
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