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Anti-Cyclin D1 Antibody (A305166)

Western Blot - Anti-Cyclin D1 Antibody (A305166) - Antibodies.com Immunohistochemistry - Anti-Cyclin D1 Antibody (A305166) - Antibodies.com
$500
Product Datasheet

Rabbit polyclonal antibody to Cyclin D1 for WB and IHC.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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+1 (314) 370-6046
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Name
Anti-Cyclin D1 Antibody
Description
Rabbit polyclonal antibody to Cyclin D1.
Applications
WB, IHC
Dilutions
WB: 1:1,000, IHC: 1:50
Reactivity
Human, Rat
Immunogen
Synthetic peptide from the N-terminal of Human Cyclin D1 (1-100 aa), conjugated to Keyhole Limpet Haemocyanin (KLH)
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Peptide affinity purification.
Concentration
1 mg/ml
Molecular Weight
40 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
AI327039, B cell CLL/lymphoma 1, B cell leukemia 1, B cell lymphoma 1 protein, B-cell lymphoma 1 protein, BCL 1, BCL-1, BCL-1 oncogene, BCL1, BCL1 oncogene, ccnd1, CCND1/FSTL3 fusion gene, included, CCND1/IGHG1 fusion gene, included, CCND1/IGLC1 fusion gene, included, CCND1/PTH fusion gene, included, CCND1_HUMAN, cD1, Cyl 1, D11S287E, G1/S specific cyclin D1, G1/S-specific cyclin-D1, Parathyroid adenomatosis 1, PRAD1, PRAD1 oncogene, U21B31
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Cyclin D1 Antibody (A305166) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Cyclin D1 Antibody (A305166)
Western blot analysis of rat brain lysate showing detection of ~33.7 kDa Cyclin D1 protein using Anti-Cyclin D1 Antibody (A305166) at 1:1,000 for 2 hours at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: rat brain lysate. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at room temperature. Color Development: ECL solution for 5 minutes at room temperature. Predicted/Observed Size: ~33.7 kDa. Other Band(s): ~40 kDa due to post translational modifications.
Immunohistochemistry - Anti-Cyclin D1 Antibody (A305166) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-Cyclin D1 Antibody (A305166)
Immunohistochemistry analysis of human esophagus, fixed in formalin and paraffin-embedded. The Primary Antibody used was Anti-Cyclin D1 Antibody (A305166) at 1:50 for 30 minutes at room temperature. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.
Publishing research using Anti-Cyclin D1 Antibody (A305166)? Please let us know so that we can list the citation on this page.

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