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Anti-Cyclin D1 Antibody (A80996)

Western Blot - Anti-Cyclin D1 Antibody (A80996) - Antibodies.com Immunohistochemistry - Anti-Cyclin D1 Antibody (A80996) - Antibodies.com Immunohistochemistry - Anti-Cyclin D1 Antibody (A80996) - Antibodies.com
This product is discontinued

Anti-Cyclin D1 Antibody (A80996) has been discontinued and is no longer available.

View all Anti-Cyclin D1 Antibodies.

Name
Anti-Cyclin D1 Antibody
Description
Rabbit polyclonal antibody to Cyclin D1.
Applications
WB, IHC
Dilutions
WB: 1:500-1:1,000, IHC: 1:50-1:100
Reactivity
Human, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 151-271 of human Cyclin D1 (NP_444284.1).
Sequence
NLAAMTPHDFIEHFLSKMPEAEENKQIIRKHAQTFVALCATDVKFISNPPSMVAAGSVVAAVQGLNLRSPNNFLSYYRLTRFLSRVIKCDPDCLRACQEQIEALLESSLRQAQQNMDPKAA
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
34 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
AI327039, B cell CLL/lymphoma 1, B cell leukemia 1, B cell lymphoma 1 protein, B-cell lymphoma 1 protein, BCL 1, BCL-1, BCL-1 oncogene, BCL1, BCL1 oncogene, ccnd1, CCND1/FSTL3 fusion gene, included, CCND1/IGHG1 fusion gene, included, CCND1/IGLC1 fusion gene, included, CCND1/PTH fusion gene, included, CCND1_HUMAN, cD1, Cyl 1, D11S287E, G1/S specific cyclin D1, G1/S-specific cyclin-D1, Parathyroid adenomatosis 1, PRAD1, PRAD1 oncogene, U21B31
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Cyclin D1 Antibody (A80996) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Cyclin D1 Antibody (A80996)
Western blot analysis of extracts of rat kidney, using Anti-Cyclin D1 Antibody (A80996) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry - Anti-Cyclin D1 Antibody (A80996) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-Cyclin D1 Antibody (A80996)
Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-Cyclin D1 Antibody (A80996) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-Cyclin D1 Antibody (A80996) - Antibodies.comEnlarge Image
Figure 3: Immunohistochemistry - Anti-Cyclin D1 Antibody (A80996)
Immunohistochemistry analysis of paraffin-embedded human esophagus using Anti-Cyclin D1 Antibody (A80996) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Publishing research using Anti-Cyclin D1 Antibody (A80996)? Please let us know so that we can list the citation on this page.

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