Rabbit polyclonal antibody to Cyclin E1 (phospho Thr395).
Specificity
This antibody detects endogenous levels of Cyclin E1 only when phosphorylated at Thr395.
Applications
WB, IHC, ELISA
Dilutions
WB: 1:500-1:1000, IHC: 1:50-1:100, ELISA: 1:5000
Reactivity
Human
Immunogen
Synthetic peptide derived from human Cyclin E1 around the phosphorylation site of Thr395 (amino acids 361-410).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Molecular Weight
47kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
CCNE, Ccne1, CCNE1_HUMAN, cyclin E variant ex5del, cyclin E variant ex7del, Cyclin Es, Cyclin Et, CyclinE, G1/S specific cyclin E, G1/S-specific cyclin-E1
Figure 1: Western Blot - Anti-Cyclin E1 (phospho Thr395) Antibody (A94475)
Western blot analysis of lysates from HeLa cells treated with Paclitaxel 1uM 60' using Anti-Cyclin E1 (phospho Thr395) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Anti-Cyclin E1 (phospho Thr395) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
![Western Blot - Anti-Cyclin E1 Antibody [ARC51383] (A305272) - Antibodies.com](https://cdn.antibodies.com/image/catalog/305/A305272_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-Cyclin E1 Antibody [rCCNE1/4936] (A277931) - Antibodies.com](https://cdn.antibodies.com/image/catalog/277/A277931_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-Cyclin E1 Antibody [rCCNE1/4936] - BSA and Azide free (A278519) - Antibodies.com](https://cdn.antibodies.com/image/catalog/278/A278519_1.jpg?profile=product_alternative)
![Western Blot - Anti-Cyclin E1 Antibody [ARC51384 + ARC51385] (A305274) - Antibodies.com](https://cdn.antibodies.com/image/catalog/305/A305274_1.jpg?profile=product_alternative)
![Western Blot - Anti-Cyclin E1 (phospho Thr77) Antibody [ARC1559] (A306326) - Antibodies.com](https://cdn.antibodies.com/image/catalog/306/A306326_1.jpg?profile=product_alternative)
