Anti-Cystatin A Antibody [ARC1993] (A309340)

Name: Anti-Cystatin A Antibody [ARC1993]
Brand: Antibodies.com
SKU: A309340

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This product is discontinued

Anti-Cystatin A Antibody [ARC1993] (A309340) has been discontinued and is no longer available.

View all Anti-Cystatin A Antibodies.

Specifications

Name

Anti-Cystatin A Antibody [ARC1993]

Description

Rabbit monoclonal [ARC1993] antibody to Cystatin A.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human Cystatin A.

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1993

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

13 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

AREI, cstA, Cystatin AS, Cystatin-A, Cystatin-AS, CYTA_HUMAN, Stefin A, Stefin-A, STF1, STFA

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-Cystatin A Antibody [ARC1993] (A309340)

Western blot analysis of extracts of various cell lines, using Anti-Cystatin A Antibody [ARC1993] (A309340) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.

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Figure 2: Immunohistochemistry - Anti-Cystatin A Antibody [ARC1993] (A309340)

Immunohistochemistry analysis of paraffin-embedded rat lung using Anti-Cystatin A Antibody [ARC1993] (A309340) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-Cystatin A Antibody [ARC1993] (A309340)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-Cystatin A Antibody [ARC1993] (A309340) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 4: Immunofluorescence - Anti-Cystatin A Antibody [ARC1993] (A309340)

Immunofluorescence analysis of NIH-3T3 cells using Anti-Cystatin A Antibody [ARC1993] (A309340) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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